The column was washed several times with TBS and 0.1 x TBS to remove any unbound molecules. Finally the column was eluted with 0.1M glycine-Cl pH2.5. 6ml of this 0.1M glycine-Cl pH2.5 was added to the column, and each sequential 800μl was collected separately. There were two guinea pigs murdered for this experiment designated 13…